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HSPA8 Monoclonal Antibody

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产品详情相关产品

HSPA8 Monoclonal Antibody

货号：

HYK-200022
规格：
图片：
Western Blot

Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate
All lanes HSPA8 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 70~75 KDa
Observed band size: 70~75 KDa
Exposure time: 5min

Western Blot
Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg
All lanes: HSPA8 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 70~75 KDa
Observed band size: 70~75 KDa
Exposure time: 5min

Western Blot
Positive WB detected in: 20μg Hela whole cell lysate
HSPA8 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 70~75 KDa
Observed band size: 70~75 KDa
Exposure time: 5min

IHC image of CSB-MA010829A1m diluted at 1:220 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA010829A1m diluted at 1:220 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-MA010829A1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Immunofluorescence staining of MCF-7 cells with CSB-MA010829A1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

产品详情

产品名称：

Mouse anti-Homo sapiens (Human) HSPA8 Monoclonal antibody
Uniprot No.：

P11142
基因名：

HSPA8
别名：

2410008N15Rik antibody; Constitutive heat shock protein 70 antibody; Epididymis luminal protein 33 antibody; Epididymis secretory sperm binding protein Li 72p antibody; Heat shock 70 kDa protein 8 antibody; Heat shock 70kD protein 10 antibody; Heat shock 70kD protein 8 antibody; Heat shock 70kDa protein 8 antibody; Heat shock cognate 71 kDa protein antibody; Heat shock cognate protein 54 antibody; Heat shock cognate protein 71 kDa antibody; Heat shock protein 8 antibody; Heat shock protein A8 antibody; Heat shock protein family A (Hsp70) member 8 antibody; Heat-shock70-KD protein 10, formerly antibody; HEL 33 antibody; HEL S 72p antibody; HSC54 antibody; HSC71 antibody; Hsc73 antibody; HSP71 antibody; HSP73 antibody; HSP7C_HUMAN antibody; HSPA10 antibody; HSPA8 antibody; LAP1 antibody; Lipopolysaccharide associated protein 1 antibody; LPS associated protein 1 antibody; LPS associated protein antibody; MGC102007 antibody; MGC106514 antibody; MGC114311 antibody; MGC118485 antibody; MGC131511 antibody; MGC29929 antibody; N-myristoyltransferase inhibitor protein 71 antibody; NIP71 antibody
宿主：

Mouse
反应种属：

Human
免疫原：

Recombinant Human Heat shock cognate 71 kDa protein (2-646AA)
免疫原种属：

Homo sapiens (Human)
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

IgG1
纯化方式：

>95%, Protein G purified
克隆号：

6E4D2
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
产品给予形式：

Liquid
应用范围：

ELISA, WB, IHC, IF, FC

推荐稀释比：

Application	Recommended Dilution
WB	1:5000-1:32000
IHC	1:100-1:500
IF	1:100-1:500
FC	1:100-1:300

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or , please kindly consult your local distributors for specific delivery time.